College of Engineering Seminar

●　TOPIC
　　The Microfluidic System Integrating Surface-Enhanced Raman Spectroscopy for Antimicrobial Susceptibility Testing
　　
●　ABSTRACT
　　Sepsis is a life-threatening dysfunction of organs, usually caused by a dysregulated immune system fighting a microbial infection. To efficiently treat sepsis, a timely and correct dosage of antibiotics is extremely important. The antimicrobial susceptibility test (AST) is a general laboratory procedure to determine the effective antimicrobial for individual patients. However, current AST methods still required prolonged bacterial culture, treatment, and labor-intensive sample preparation steps. To address the above problems, we integrate the microfluidic platform with surface-enhanced Raman spectroscopy (SERS) to perform rapid, automated, and multiplex AST. Based on its automation and micro-environmental control features, the microfluidic system consisting of a series of branches and microwell array can perform on-chip bacteria sample processing steps, including enrichment, isolation, and purification. Furthermore, the system can create an antibiotic concentration gradient for high-throughput and multiplex AST and minimum inhibitory concentration (MIC) in a single chip. We envision a sample-efficient, accurate, and multiplex SERS-AST platform, allowing clinicians to monitor real-time patient conditions better, predict their prognosis, and provide timely treatments.
　　
●　SPEAKER
　　Prof. Nien-Tsu Huang,
　　Department of Electrical Engineering, National Taiwan University

●　HOST
　　Prof. Sheng-Shian Li
　　Institute of Nano-Engineering and Micro-System, National Tsing Hua University

●　TIME
　　2021/10/19 (Tue) 13:20 - 15:20

●　VENUE
　　Google Meet (LINK)