11310E500100 College of Engineering Seminar

TOPIC
▸　Development of Affordable Sensing Technologies in Liquid Biopsy Diagnostics

Abstract
❝　The advancement of liquid biopsy techniques for non-invasive tumor analysis holds immense potential in continuously tracking tumor status—recurrence, progression, and treatment response—in real-time. To comprehensively assess tumor evolution, cellular diversity, and drug resistance mechanisms, a pivotal step involves scrutinizing proteins and nucleic acids within extracellular vesicles (EVs) present in biofluids. Our focus lies in crafting an advanced digital bead-based sensor system that seamlessly evaluates the molecular profile of EVs in glioblastoma cases. Our innovative approach enriches tumor-relevant genetic data within EVs using antibody-specific magnetic beads, subsequently amplifying it via reverse transcription recombinase polymerase amplification system across forty thousand droplets. These results are scrutinized using a compact imaging device. The core strengths of our methodology include, (a) Streamlined Sample Processing: Our system facilitates ultra-sensitive miRNA detection in EVs within a rapid ninety-minute window, owing to optimized sample preparation and a compact detection mechanism; (b) Enhanced Signal Clarity: Employing a straightforward isothermal assay significantly boosts the fluorescent signal-to-noise ratio within each monosized 70-µm droplet; (c) Real-Time Data Analysis: Our system seamlessly captures real-time imaging data for the edge computing, utilizing trained YOLO models for classification, and presents genetic results on the smart device. Its user-friendly design enables utilization by healthcare workers with varying skill levels. This pioneering approach has the potential to offer affordable, rapid, and informative diagnostics particularly beneficial for underserved regions and healthcare systems facing financial constraints. Furthermore, its adaptability for diverse cancers could revolutionize the pace of diagnosis and treatment decision-making through liquid biopsy, potentially establishing its widespread application in healthcare.　❞

SPEAKER
▸　Prof. Hsing-Ying LIN (*CV)
▸　Institute of Biomedical Engineering, National Tsing Hua University

Short Bio
❝　Dr. Hsing-Ying Lin serves as an Assistant Professor in the Institute of Biomedical Engineering at National Tsing Hua University. Before relocating to NTHU, she was a Research Fellow in the Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School. She got prestigious MGH FMD fellowship (2018) and NIH T32 training grant (2019) in US. She focuses on the development of point-of-care devices with rapid streamlined assays. She has strong interests in the liquid biopsy arena, developing several integrated sensing platforms and streamlined signal enhancement assay methods for sensitive, high-throughput profiling of oncological extracellular vesicle markers in clinical samples for translational medicine studies. Her publishments encompass Nature Biomedical Engineering, ACS Nano, Small, Biosensor and Bioelectronics, Advanced Healthcare Materials, etc.　❞

TIME
▸　2024/09/24 (TUE) 13:20 ~ 15:10

VENUE
▸　Classroom 212, Chemical Engineering Building